{"created":"2023-06-19T10:53:51.444193+00:00","id":1990,"links":{},"metadata":{"_buckets":{"deposit":"05db57e4-1011-4edd-a2fe-e6788d5fbe1f"},"_deposit":{"created_by":3,"id":"1990","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1990"},"status":"published"},"_oai":{"id":"oai:kobe-c.repo.nii.ac.jp:00001990","sets":["40:263:265"]},"author_link":["3837","3838","3840","3839","3645"],"item_2_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"マイクロスケール ジッケン オ モチイタ キウイ ニ フクマレル タンパクシツ ブンカイ コウソ ノ キョウザイ カイハツ"}]},"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-12-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"86","bibliographicPageStart":"79","bibliographicVolumeNumber":"58","bibliographic_titles":[{"bibliographic_title":"神戸女学院大学論集"},{"bibliographic_title":"KOBE COLLEGE STUDIES","bibliographic_titleLang":"en"}]}]},"item_2_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"椎葉, 昌美"},{"creatorName":"シイバ, マサミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3837","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"土師,  麻里奈"},{"creatorName":"ハゼ, マリナ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3838","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久野, 香月"},{"creatorName":"ヒサノ, カヅキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"水野, 暢子"},{"creatorName":"ミズノ, ノブコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3840","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中川, 徹夫"},{"creatorName":"ナカガワ, テツオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3645","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070312866","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000070312866"},{"nameIdentifier":"70312866","nameIdentifierScheme":"KAKEN-研究者検索","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000070312866/"}]}]},"item_2_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHIIBA, Masami","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3837","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HAZE, Marina","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3838","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HISANO, Kaduki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MIZUNO, Nobuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3840","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"NAKAGAWA, Testuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3645","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070312866","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000070312866"},{"nameIdentifier":"70312866","nameIdentifierScheme":"KAKEN-研究者検索","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000070312866/"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"要旨（日）","attribute_value_mlt":[{"subitem_description":"Various physicochemical properties of enzymes such as structures, reactions, thermodynamics, kinetics, and so forth appear in Japanese high school biology and chemistry textbooks. Glass petri dishes and pudding cups are usually used in traditional high school experiments concerning enzymes. However, the traditional methods need many reagents and extended experiment time. In this paper alternative microscale methods using 6-well plates are proposed, and the temperature effect and substrate specificity of proteases contained in Kiwifruits, which are mainly composed of actinidin, have been investigated. Both the amount of samples or reagents and the experiment time in our methods are drastically reduced in comparison with those in the traditional approaches. Moreover, our microscale methods enable each student to perform the experiments by him/herself. Therefore, it has been shown that they are very useful as teaching materials for high school biology and chemistry.","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18878/00001951","subitem_identifier_reg_type":"JaLC"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00085725","subitem_source_identifier_type":"NCID"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"神戸女学院大学人間科学部環境・バイオサイエンス学科"},{"subitem_text_value":"神戸女学院大学人間科学部環境・バイオサイエンス学科"},{"subitem_text_value":"神戸女学院大学人間科学部環境・バイオサイエンス学科"},{"subitem_text_value":"神戸女学院大学人間科学部環境・バイオサイエンス学科"},{"subitem_text_value":"神戸女学院大学人間科学部環境・バイオサイエンス学科"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2011-12-01"}],"displaytype":"detail","filename":"KJ00007675179.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","license_note":"神戸女学院大学研究所","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00007675179","url":"https://kobe-c.repo.nii.ac.jp/record/1990/files/KJ00007675179.pdf"},"version_id":"d27559bc-e8ec-4dfd-951d-424f8f45a7ad"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"マイクロスケール実験","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質分解酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"教材","subitem_subject_scheme":"Other"},{"subitem_subject":"高校生物","subitem_subject_scheme":"Other"},{"subitem_subject":"高校化学","subitem_subject_scheme":"Other"},{"subitem_subject":"microscale experiment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"teaching materials","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high school biology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high school chemistry","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マイクロスケール実験を用いたキウイに含まれるタンパク質分解酵素の教材開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マイクロスケール実験を用いたキウイに含まれるタンパク質分解酵素の教材開発"},{"subitem_title":"The Development of Teaching Materials using Microscale Experiments to Examine Protease Contained in Kiwifruits","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["265"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-12-01"},"publish_date":"2011-12-01","publish_status":"0","recid":"1990","relation_version_is_last":true,"title":["マイクロスケール実験を用いたキウイに含まれるタンパク質分解酵素の教材開発"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T11:23:31.146198+00:00"}