{"created":"2024-02-05T04:20:02.099326+00:00","id":2000148,"links":{},"metadata":{"_buckets":{"deposit":"75fe46ae-e05f-4539-8d0e-aa943d293151"},"_deposit":{"created_by":21,"id":"2000148","owner":"21","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"2000148"},"status":"published"},"_oai":{"id":"oai:kobe-c.repo.nii.ac.jp:02000148","sets":["40:1693203673259:1693203904796"]},"author_link":["6069","6065","6070"],"control_number":"2000148","item_10002_biblio_info_30":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-12-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"41","bibliographicPageStart":"33","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"神戸女学院大学論集","bibliographic_titleLang":"ja"},{"bibliographic_title":"KOBE COLLEGE STUDIES","bibliographic_titleLang":"en"}]}]},"item_10002_description_28":{"attribute_name":"要旨(日）","attribute_value_mlt":[{"subitem_description":"本研究は本研究は、血液細胞からの赤血球分化の実験モデルとして慢性骨髄性白血病細胞株 K562 を使用して、赤血球分化におけるクロマチン制御分子 SATB1 の役割を明らかにすることを目的としている。SATB1 の機能を明らかにするため、K562 における SATB1 ノックアウトを行った。SATB1 ノックアウト K562 細胞は、親株の K562 細胞と同様の細胞増殖能を示した。RNA-Seq 法を用いた遺伝子発現比較解析では、GO エンリッチメント解析の結果、「分子機能」のカテゴリーにおいて、SATB1 ノックアウトが、“binding” および、“protein binding” に関わる遺伝子群を特に変動させていることが分かった。さらに、KEGG エンリッチメント解析では、遺伝子発現変化が見られた細胞内パスウェイの中に、今回の SATB1 プロジェクトと関連の大きい “Hematopoietic cell lineage”（造血細胞の分化系統）が含まれていた。今回の RNA-Seq 解析の結果は、造血細胞の分化における SATB1 の重要性を示唆するものだった。","subitem_description_type":"Other"}]},"item_10002_description_49":{"attribute_name":"要旨（英）","attribute_value_mlt":[{"subitem_description":"We created SATB1-knockout clones of K562 cells through genome editing. These knockout cells showed similar proliferation activity as the wild-type K562 cells in the in vitro culture assays. Gene Ontology (GO) enrichment analysis using RNA sequencing revealed that SATB1-deletion changed expression of genes associated with “Molecular Function” category of GO. KEGG pathway enrichment analysis showed that SATB1-deletion significantly changed expression of genes associated with “Hematopoietic cell lineage” pathway. These results suggest that SATB1 plays important roles in hematopoietic cell differentiation.","subitem_description_type":"Other"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18878/0002000148","subitem_identifier_reg_type":"JaLC"}]},"item_10002_select_43":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_10002_text_31":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"神戸女学院大学研究所"}]},"item_10002_text_47":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 教授"},{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 4年生"},{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 4年生"},{"subitem_text_value":"神戸女学院大学大学院 人間科学研究科 博士前期課程"},{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 嘱託教学職員"}]},"item_10002_text_48":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_text_value":"AN00085725"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"佐藤, 友亮","creatorNameLang":"ja"},{"creatorName":"サトウ, ユウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"SATOH, Yusuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"佐藤","familyNameLang":"ja"},{"familyName":"サトウ","familyNameLang":"ja-Kana"},{"familyName":"SATOH","familyNameLang":"en"}],"givenNames":[{"givenName":"友亮","givenNameLang":"ja"},{"givenName":"ユウスケ","givenNameLang":"ja-Kana"},{"givenName":"Yusuke","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6065","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松尾, 亜美","creatorNameLang":"ja"},{"creatorName":"MATSUO, Ami","creatorNameLang":"en"},{"creatorName":"マツオ, アミ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"森田, 来実","creatorNameLang":"ja"},{"creatorName":"MORITA, Kurumi","creatorNameLang":"en"},{"creatorName":"モリタ, クルミ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"大平, 直子","creatorNameLang":"ja"},{"creatorName":"OHIRA, Naoko","creatorNameLang":"en"},{"creatorName":"オオヒラ, ナオコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"6069","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高比良, 響","creatorNameLang":"ja"},{"creatorName":"TAKAHIRA Hibiki","creatorNameLang":"en"},{"creatorName":"タカヒラ, ヒビキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"6070","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-05"}],"displaytype":"detail","fileDate":[{"fileDateValue":"2024-02-05"}],"filename":"202312_191-04.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","license_note":"神戸女学院大学研究所","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://kobe-c.repo.nii.ac.jp/record/2000148/files/202312_191-04.pdf"},"version_id":"98aa1ade-8263-4252-9702-d1085e7471c4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SATB1","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA-Seq","subitem_subject_scheme":"Other"},{"subitem_subject":"K562","subitem_subject_scheme":"Other"},{"subitem_subject":"慢性骨髄性白血病","subitem_subject_scheme":"Other"},{"subitem_subject":"SATB1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA-Sequencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"K562","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Chronic Myeloid Leukemia","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"RNA-Seq 法による SATB1 ノックアウト K562 細胞の遺伝子発現解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"RNA-Seq 法による SATB1 ノックアウト K562 細胞の遺伝子発現解析","subitem_title_language":"ja"},{"subitem_title":"Gene Expression Analyses of SATB1-Knockout K562 Cells using RNA-Sequencing","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["1693203904796"],"pubdate":{"attribute_name":"公開日","attribute_value":"2024-02-05"},"publish_date":"2024-02-05","publish_status":"0","recid":"2000148","relation_version_is_last":true,"title":["RNA-Seq 法による SATB1 ノックアウト K562 細胞の遺伝子発現解析"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-04-19T05:57:24.765501+00:00"}