{"created":"2023-06-19T10:55:05.555409+00:00","id":5614,"links":{},"metadata":{"_buckets":{"deposit":"f0b4c965-02dc-4093-ada1-e9679d672fee"},"_deposit":{"created_by":17,"id":"5614","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"5614"},"status":"published"},"_oai":{"id":"oai:kobe-c.repo.nii.ac.jp:00005614","sets":["40:1403:1410"]},"author_link":["5971","3869","5792"],"item_10002_biblio_info_30":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-12-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"9","bibliographicPageStart":"1","bibliographicVolumeNumber":"65","bibliographic_titles":[{"bibliographic_title":"神戸女学院大学論集"},{"bibliographic_title":"KOBE COLLEGE STUDIES","bibliographic_titleLang":"en"}]}]},"item_10002_description_28":{"attribute_name":"要旨(日）","attribute_value_mlt":[{"subitem_description":"明石川水系に生息するメダカについて、在来集団の遺伝子型分布および外来集団の移入による遺伝的攪乱の実態を明らかにするため、９地点より計65尾のメダカを捕獲し、ミトコンドリアDNAを用いた遺伝子型を解析すると共に、ヒメダカ体色原因遺伝子（bマーカ）を指標にしたヒメダカ遺伝子移入の有無を調査した。遺伝子型分布については、8地点で捕獲した62尾の遺伝子型がいずれもB1aであったことから、明石川水系の野生集団は瀬戸内沿岸在来の遺伝子型B1aのみで構成されていることが示唆された。しかしながら、中流域の２地点で各々捕獲した1尾、計2尾からは東日本在来の遺伝子型B27が検出され、さらに中流域の1地点で捕獲した1尾からはヒメダカ遺伝子の移入が確認された。以上の結果から、明石川水系のメダカは、全体を通してみた場合、単一の遺伝子からなる在来集団であるが、中流域に生息する一部の在来集団に遺伝的攪乱が生じていることが示唆された。","subitem_description_type":"Other"}]},"item_10002_description_49":{"attribute_name":"要旨（英）","attribute_value_mlt":[{"subitem_description":"To characterize the genotype distribution of wild populations of medaka inhabiting the Akashi River system and to determine the actual state of genetic disturbance due to ingression of adventive populations, we conducted a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome b gene and PCR analysis of the causative gene for the orange-red strain (hi-medaka), respectively. In total, we collected 65 fishes from 9 sites, 62 of which from 8 sites had the B1a mitotype, which is a native genotype belonging to the Setouchi subgroup and is found at various locations within Hyogo Prefecture. This result suggests that the native populations of medaka in the Akashi River system comprise only a single genotype. However, 2 fishes from 2 sites in the middle basin had the B27 mitotype, which belongs to the Higashi-nihon Ⅱsubgroup and, therefore, this fish did not originally inhabit the Akashi River system. Moreover, genetic introduction was confirmed in 1 fish from a site in the middle basin, as evidenced by its hi-medaka sequence. In summary, the Akashi River system comprises a local population of wild medaka that constitutes a single genotype. However, this study suggests that genetic disturbance has occurred in certain medaka populations in the middle basin.","subitem_description_type":"Other"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18878/00005555","subitem_identifier_reg_type":"JaLC"}]},"item_10002_select_43":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_10002_text_31":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_text_value":"神戸女学院大学研究所"}]},"item_10002_text_47":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"神戸女学院大学 人間科学部環境・バイオサイエンス学科 嘱託教学職員"},{"subitem_text_value":"神戸女学院大学 人間科学部環境・バイオサイエンス学科 卒業生"},{"subitem_text_value":"神戸女学院大学 人間科学部環境・バイオサイエンス学科 准教授"}]},"item_10002_text_48":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_text_value":"AN00085725"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"江口, さやか"},{"creatorName":"エグチ, サヤカ","creatorNameLang":"ja-Kana"},{"creatorName":"EGUCHI, Sayaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"文木, 春菜"},{"creatorName":"フミキ, ハルナ","creatorNameLang":"ja-Kana"},{"creatorName":"FUMIKI, Haruna","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5971","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"横田, 弘文"},{"creatorName":"ヨコタ, ヒロフミ","creatorNameLang":"ja-Kana"},{"creatorName":"YOKOTA, Hirofumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5792","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-01-08"}],"displaytype":"detail","filename":"201812_181-02.pdf","filesize":[{"value":"646.4 kB"}],"format":"application/pdf","license_note":"神戸女学院大学研究所","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"201812_181-02","url":"https://kobe-c.repo.nii.ac.jp/record/5614/files/201812_181-02.pdf"},"version_id":"7eca170a-d7b5-4116-b416-e4e2ded36871"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"遺伝子型分析","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子移入","subitem_subject_scheme":"Other"},{"subitem_subject":"国内外来種","subitem_subject_scheme":"Other"},{"subitem_subject":"チトクロームb遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR-RFLP","subitem_subject_scheme":"Other"},{"subitem_subject_scheme":"Other"},{"subitem_subject":"genotypic analyses","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"introgression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"domestic alien species","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytochrome b gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR-RFLP","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"明石川水系に生息するメダカの遺伝子型分布および遺伝的攪乱","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"明石川水系に生息するメダカの遺伝子型分布および遺伝的攪乱"},{"subitem_title":"Genotype Distribution and Genetic Disturbance in Wild Populations of Medaka Inhabiting the Akashi River System","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"17","path":["1410"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-01-08"},"publish_date":"2019-01-08","publish_status":"0","recid":"5614","relation_version_is_last":true,"title":["明石川水系に生息するメダカの遺伝子型分布および遺伝的攪乱"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-06-19T11:30:57.465463+00:00"}