{"created":"2023-06-19T10:55:22.526203+00:00","id":6042,"links":{},"metadata":{"_buckets":{"deposit":"84e996c3-f226-448b-8d86-8bcb0a5bb163"},"_deposit":{"created_by":21,"id":"6042","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"6042"},"status":"published"},"_oai":{"id":"oai:kobe-c.repo.nii.ac.jp:00006042","sets":["40:1449:1478"]},"author_link":["6068","6075","6072","6073","6074","6066","6070","6065","6076","6067","6069"],"item_10002_biblio_info_30":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-12-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"57","bibliographicPageStart":"49","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"神戸女学院大学論集"},{"bibliographic_title":"KOBE COLLEGE STUDIES","bibliographic_titleLang":"en"}]}]},"item_10002_description_28":{"attribute_name":"要旨(日）","attribute_value_mlt":[{"subitem_description":"　本研究は、赤血球分化におけるクロマチン制御分子 SATB1 の役割を明らかにすることを目的としている。血液細胞からの赤血球分化の実験モデルとして、慢性骨髄性白血病細胞株 K562 を使用することとした。本稿では、この研究プロジェクトの開始に際して行った K562 細胞の基礎的研究について報告する。ヘミンによる K562 細胞の赤血球系への分化誘導条件を検討した。その結果、ヘミン濃度 60 mM が、30 mM よりもより有効に赤血球系への分化を誘導することが明らかになった。また、イマチニブによる K562 細胞の増殖に与える影響を検討したところ、イマチニブ濃度 1 mM および10 mM の双方において、K562 細胞の強い増殖抑制が認められた。SATB1 の赤血球分化における機能を明らかにするため、ゲノム編集技術を用いて K562 における SATB1 ノックアウトを試みた。リアルタイム RT-PCR 法を用いて、複数のノックアウトクローンにおける SATB1 の発現確認を行ったが、SATB1 の遺伝子欠失を確認できなかった。今後、SATB1 ノックアウト K562 細胞を樹立し、赤血球分化における SATB1 の機能を解析する予定である。","subitem_description_type":"Other"}]},"item_10002_description_49":{"attribute_name":"要旨（英）","attribute_value_mlt":[{"subitem_description":"　We previously identified a special AT-rich sequence-binding protein 1 (SATB1)— a global chromatin organizer, as a lymphoid-lineage-inducing gene in Hematopoietic stem cells. SATB1 is involved in the tissue-specific organization of DNA sequences, regulation of gene expression, and designation of region- specific histone modifications. Moreover, during early erythroid development, SATB1 may regulate the expression of globin genes. Therefore, we launched a new research project exploring the roles of SATB1 in erythroid differentiation using the chronic myeloid leukemia cell line K562. First, we evaluated the fundamental traits of K562 cells and confirmed that K562 cells more readily differentiated into erythroid cells at a hemin concentration of 60 mM than of 30 mM. Next, we assessed the effects of a tyrosine kinase inhibitor, imatinib, on K562 cell proliferation in vitro. At imatinib concentrations of 1 and 10 mM, K562 cell proliferation was markedly suppressed. Currently, we are attempting to establish SATB1-knockout clones of K562 cells through genome editing. In future research, we intend to evaluate the roles of SATB1 in erythroid differentiation using SATB1-knockout K562 cells.","subitem_description_type":"Other"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18878/00005944","subitem_identifier_reg_type":"JaLC"}]},"item_10002_select_43":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_10002_text_31":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_text_value":"神戸女学院大学研究所"}]},"item_10002_text_47":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 教授"},{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 4年生"},{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 4年生"},{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 4年生"},{"subitem_text_value":"神戸女学院大学大学院　人間科学研究科　博士前期課程"},{"subitem_text_value":"神戸女学院大学 人間科学部 環境・バイオサイエンス学科 嘱託教学職員"}]},"item_10002_text_48":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_text_value":"AN00085725"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"佐藤, 友亮","creatorNameLang":"ja"},{"creatorName":"サトウ, ユウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"SATOH, Yusuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"佐藤","familyNameLang":"ja"},{"familyName":"サトウ","familyNameLang":"ja-Kana"},{"familyName":"SATOH","familyNameLang":"en"}],"givenNames":[{"givenName":"友亮","givenNameLang":"ja"},{"givenName":"ユウスケ","givenNameLang":"ja-Kana"},{"givenName":"Yusuke","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6065","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"浅沼, 萌花"},{"creatorName":"アサヌマ, モエカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"6066","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大辻, 瑠倫"},{"creatorName":"オオツジ, ルリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"6067","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塚本, 皐月"},{"creatorName":"ツカモト, サツキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"6068","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大平, 直子"},{"creatorName":"オオヒラ, 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Yusuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"佐藤","familyNameLang":"ja"},{"familyName":"サトウ","familyNameLang":"ja-Kana"},{"familyName":"SATOH","familyNameLang":"en"}],"givenNames":[{"givenName":"友亮","givenNameLang":"ja"},{"givenName":"ユウスケ","givenNameLang":"ja-Kana"},{"givenName":"Yusuke","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6065","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ASANUMA, Moeka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6072","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OTSUJI, Ruri","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6073","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TSUKAMOTO, Satsuki ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6074","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OHIRA, Naoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6075","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TAKAHIRA, Hibiki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6076","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-01-13"}],"displaytype":"detail","filename":"202212_189-05.pdf","filesize":[{"value":"539.1 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Leukemia","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"白血病細胞株 K562 を用いた赤血球分化に関する基礎的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"白血病細胞株 K562 を用いた赤血球分化に関する基礎的研究"},{"subitem_title":"Fundamental Traits of K562 for the Study of SATB1-Induced Erythroid Differentiation","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["1478"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-01-13"},"publish_date":"2023-01-13","publish_status":"0","recid":"6042","relation_version_is_last":true,"title":["白血病細胞株 K562 を用いた赤血球分化に関する基礎的研究"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-04-19T05:57:25.169673+00:00"}